当前位置: 首页 / 科学研究 / 研究成果 / 正文

MPK6 controls H2O2-induced root elongation by ......

2014年09月19日 17:19  点击:[]

MPK6 controls H2O2 -induced root elongation by mediating Ca2+  influx across the plasma membrane of root cells in Arabidopsis seedlings
作     者:Shuan Han, Lin Fang, Xuejian Ren, Wenle Wang and Jing Jiang
刊物名称: New Phytologist
出版年份: 2014
卷:   期:   页码:
文章摘要 :
Mitogen-activated protein kinases (MPKs) play critical roles in signalling and growth, and Ca2+ and H2O2 control plant growth processes associated with abscisic acid (ABA). However, it remains unclear how MPKs are involved in  H2O2 - and Ca2+  -mediated root elongation.
Root elongation in seedlings of the loss-of-function mutant Atmpk6 (Arabidopsis thaliana MPK6) was less sensitive to moderate  H2O2 or ABA than that in wild-type (WT) plants. The enhanced elongation was a result of root cell expansion. This effect disappeared when ABAinduced  H2O2 accumulation or the cytosolic Ca2+  increase were defective.
Molecular and biochemical evidence showed that increased expression of the cell wall peroxidase PRX34 in Atmpk6 root cells enhanced apoplastic  H2O2 generation; this promoted a cytosolic Ca2+  increase and Ca2+   influx across the plasma membrane. The plasma membrane damage caused by high levels of  H2O2 was ameliorated in a Ca2+ -dependent manner.
These results suggested that there was intensified PRX34-mediated  H2O2 generation in the apoplast and increased Ca2+  flux into the cytosol of Atmpk6 root cells; that is, the spatial separation of apoplastic H2O2 from cytosolic Ca2+  in root cells prevented  H2O2 -induced inhibition of root elongation in Atmpk6 seedlings.




Copyright@2017  棉花生物学国家重点实验室  植物逆境生物学实验室  All Rights reserved

地址:中国-河南-开封 联系电话:0371 — 23881387 传真:0371 — 23881387

 技术支持:河南大学网站工作