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MPK6 controls H2O2- induced root elongation by.........

2016年03月10日 17:25  点击:[]

题目:MPK6 controls H 2O 2- induced root elongation by mediating Ca 2+influx across the plasma membrane of root cells in Arabidopsi seedlings
刊物名称: New Phytol
出版年份: 2015
卷:205,期:2, 页码:695-706
Keywords: AtMPK6; abscisic acid (ABA); apoplastic H2O2 increase; cell wall peroxidase34; cytosolic C 2+ rise; plasma membrane Ca2+ channels; root elongation
Abstract Mitogen-activated protein kinases (MPKs) play critical roles in signalling and growth, and Ca(2+) and H 2 O 2 control plant growth processes associated with abscisic acid (ABA). However, it remains unclear how MPKs are involved in H 2 O 2 - and Ca(2+) -mediated root elongation. Root elongation in seedlings of the loss-of-function mutant Atmpk6 (Arabidopsis thaliana MPK6) was less sensitive to moderate H 2 O 2 or ABA than that in wild-type (WT) plants. The enhanced elongation was a result of root cell expansion. This effect disappeared when ABA-induced H 2 O 2 accumulation or the cytosolic Ca(2+) increase were defective. Molecular and biochemical evidence showed that increased expression of the cell wall peroxidase PRX34 in Atmpk6 root cells enhanced apoplastic H 2O 2 generation; this promoted a cytosolic Ca(2+) increase and Ca(2+) influx across the plasma membrane. The plasma membrane damage caused by high levels of H 2 O 2 was ameliorated in a Ca(2+) -dependent manner. These results suggested that there was intensified PRX34-mediated H2 O2 generation in the apoplast and increased Ca(2+) flux into the cytosol of Atmpk6 root cells; that is, the spatial separation of apoplastic H 2 O 2 from cytosolic Ca(2+) in root cells prevented H 2 O 2 -induced inhibition of root elongation in Atmpk6 seedlings.

http://onlinelibrary.wiley.com/doi/10.1111/nph.12990/abstract;jsessionid=348A03BA99BD05B874FCD93D76417CEF.f03t01




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