题目：Screening and analysis of rhizosphere acidification deficiency mutants in Arabidopsis thaliana under low phosphorus作者：Kai Jian Lei, Jun Yan Xie, Yuan Yuan Zhu, Chun Peng Song & Guo Yong An刊物名称: Soil Science and Plant Nutrition出版年份: 2015卷:61 期:3 页码:1-8Keywords:   Abstract：During plant development and growth, low phosphorus (P) is a common stress. Under low P, plant roots release organic acids to acidify the rhizosphere, which is an important physiological response of plants during adaptation to low-P stress. Acidification enhances the solubility of P in the rhizosphere and increases P uptake by plants. However, how plants perceive and transduce the signal of low P, and acidify the rhizosphere to achieve efficient absorption and utilization of P, is not clear. This report established a direct and efficient method to screen rhizosphere acidification deficiency mutants by observing the color change on medium containing bromocresol purple. Twenty-two rhizosphere acidification deficiency mutants were cloned from a library of 70000 T-DNA insertion mutants. These mutants were related to, for example, transcription regulation, calcium ions and Proton-Adenosine Triphosphatase (H+-ATPase), which represent starting points for understanding the mechanism for the regulation of P nutrition, absorption and utilization via rhizosphere acidification.http://xueshu.baidu.com/s?wd=paperuri%3A%28ff6f74b82e0950c42656a0107b961908%29&filter=sc_long_sign&tn=SE_xueshusource_2kduw22v&sc_vurl=http%3A%2F%2Fwww.tandfonline.com%2Fdoi%2Ffull%2F10.1080%2F00380768.2015.1007025&ie=utf-8