E-mail：zhuxiaohong@henu.edu.cn
朱晓红，女，博士，教授，博士生导师。1990年获扬州大学理学硕士学位，2003年获以色列魏兹曼研究所理学博士学位。2003-2008年在美国耶鲁大学从事博士后研究，主要从事植物生物胁迫适应机制研究。2008-2010年和2011-2017分别在美国密西根技术大学和普渡大学担任研究助理教授和研究科学家，主要从事植物逆境胁迫的应答机制研究。在Plant Cell, EMBO J, Cell Host & Microbe, Molecular Plant and Plant Physiology 等国际知名学术期刊发表研究论40篇。
主要研究方向
1.植物线粒体反向调控机制
线粒体提供细胞活动的能量, 直接应答外界刺激，并反向调控细胞核基因的表达 (Mitochondrial Retrograde Regulation，MRR)。线粒体反向调控信号途径，通过感知线粒体的功能状态，建立线粒体与细胞核之间的信息交流，重新编程线粒体的代谢，维持细胞的稳态 (Homeostasis)以适应内外环境的变化。我们通过遗传学，细胞学，生物化学和分子生物学的手段，试图解析线粒体反向调控机制及其在植物逆境适应中的作用。
2.钙离子介导的信号传导在调控植物衰老和逆境感应中的作用
细胞质中钙离子浓度的变化是生物体在感知内外环境变化时，产生的一种快速地信号应答反应。钙离子浓度升高的幅度，频率和持续的时间构成一种植物细胞应答内外刺激的“钙语言”，可以被细胞质中特定蛋白质解读，并递给细胞核重设基因的表达来调节细胞功能。所以，理解钙信号的发生，传导及其对基因表达的调控，可以为提高植物逆境适应性提供理论基础。我们特别感兴趣的是线粒体 “钙语言”在调控植物衰老和逆境适应中的作用。我们通过遗传学，分子生物学，细胞生物学以及钙离子成像技术， 揭示线粒体钙离子稳态平衡的调控。
主持的研究项目
1. 国家基金面上项目： 组蛋白甲基化修饰酶在线粒体反向调控中的作用 （2019/01-2022/12）
代表性研究论文和著作 (*为通讯作者)：
1. Wang W, Chen Q, Xu S, Liu WC, Zhu X, Song CP. Trehalose-6-phosphate phosphatase E modulates ABA-controlled root growth and stomatal movement in Arabidopsis. J Integr Plant Biol. 2020 Mar 13. doi: 10.1111/jipb.12925.
2. Zhao H, Zhang Y, Pan M, Song Y, Bai L, Miao Y, Huang Y, Zhu X, Song CP. Dynamic imaging of cellular pH and redox homeostasis with a genetically encoded dual-functional biosensor, pHaROS, in yeast. J Biol Chem. 2019 Oct 25;294(43):15768-15780. doi: 10.1074/jbc.
3. Wu WQ, Zhu X, Song CP. Single-molecule technique: a revolutionary approach to exploring fundamental questions in plant science. New Phytol. 2019 Jul; 223(2):508-510. doi: 10.1111/nph.15928.
4. Sun L, Xu Y, Bai S, Bai X, Zhu H, Dong H, Wang W, Zhu X, Hao F, Song CP. Transcriptome-wide analysis of pseudouridylation of mRNA and non-coding RNAs in Arabidopsis. J Exp Bot. 2019 Jun 6. pii: erz273. doi: 10.1093/jxb/erz273.
5. Dong H, Bai L, Zhang Y, Zhang G, Mao Y, Min L, Xiang F, Qian D, Zhu X, Song CP. Modulation of Guard Cell Turgor and Drought Tolerance by a Peroxisomal Acetate–Malate Shunt. Molecular Plant. 2018, DOI:10.1016/j.molp.2018.07.008.
6. Zhu X*, Taylor A, Zhang S, Zhang D, Feng Y and Liang G and Zhu JK*. Measuring spatial and temporal Ca2+ signals in Arabidopsis plants. JoVE. 2014, Sep 2; (91). doi: 10.3791/51945 (*corresponding authors).
7. Zhu X and Zhu JK. Double repression in jasmonate-mediated plant defense. Molecular Cell. 2013, 50(4): 459-460.
8. Zhu X*, Feng Y, Liang G, Liu N, Zhu JK*. Aequorin-based luminescence imaging reveals stimulus- and tissue-specific Ca2+ dynamics in Arabidopsis plants. Molecular Plant. 2013, 6(2): 444-455 (*corresponding authors).
9. Yan J, Wang P, Wang B, Hsu CC, Tang K, Zhang H, Hou YJ, Zhao Y, Wang Q, Zhao C, Zhu X, Tao WA, Li J, Zhu JK. The SnRK2 kinases modulate miRNA accumulation in Arabidopsis. PLoS Genet. 2017 Apr 18;13(4): e1006753.
10. Yan J, Zhao C, Zhou J, Yang Y, Wang P, Zhu X, Tang G, Bressan RA, Zhu JK. The miR165/166 Mediated Regulatory Module Plays Critical Roles in ABA Homeostasis and Response in Arabidopsis thaliana. PLoS Genet. 2016 Nov 3; 12(11): e1006416.
11. Hou YJ, Zhu Y, Wang P, Zhao Y, Xie S, Batelli G, Wang B, Duan CG, Wang X, Xing L, Lei M, Yan J, Zhu X, Zhu JK. Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling. PLoS Genetics. 2016 Mar 4; 12(3): e1005835.
12. Liu W, Zhu X, Lei M, Xia Q, Botella JR, Zhu JK, Mao Y. A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana. Science Bulletin. 2015 60(15):1332-1347.
13. Li Y, Duan CG, Zhu X, Qian W, Zhu JK. A DNA ligase required for active DNA demethylation and genomic imprinting in Arabidopsis. Cell Research. 2015, Jun; 25(6):757-60.
14. Li Y, Córdoba-Cañero D, Qian W, Zhu X, Tang K, Zhang H, Ariza RR, Roldán-Arjona T, Zhu JK. An AP endonuclease functions in active DNA demethylation and gene imprinting in Arabidopsis. PLoS Genetics 2015, Jan 8;11 (1): e1004905.
15. Wang P, Du Y, Hou YJ, Zhao Y, Hsu CC, Yuan F, Zhu X, Tao WA, Song CP, Zhu JK. Nitric oxide negatively regulates abscisic acid signaling in guard cells by S-nitrosylation of OST1. PANS. 2015 Jan 13; 112 (2):613-8.
16. Duan CG, Zhang H, Tang K, Zhu X, Qian W, Hou YJ, Wang B, Lang Z, Zhao Y, Wang X, Wang P, Zhou J, Liang G, Liu N, Wang C, Zhu JK. Specific but interdependent functions for Arabidopsis AGO4 and AGO6 in RNA-directed DNA methylation. EMBO Journal. 2015 Mar 4;34 (5):581-92.
17. Wan Y, Tang K, Zhang DY, Xie S, Zhu X, Wang Z, Lang Z. Transcriptome-wide high-throughput deep m6A-seq reveals unique differential m6A methylation patterns between three organs in Arabidopsis thaliana. Genome Biology. 2015 16:272.
18. Qian W, Miki D, Lei M, Zhu X, Zhang H, Liu Y, Li Y, Lang Z, Wang J, Tang K, Liu R, Zhu JK. Regulation of Active DNA Demethylation by an α-Crystallin Domain Protein in Arabidopsis. Molecular Cell. 2014 55(3):361-371.
19. Zhao Y, Xing L, Wang X, Hou YJ, Gao J, Wang P, Duan CG, Zhu X, Zhu JK. The ABA receptor PYL8 promotes lateral root growth by enhancing MYB77-dependent transcription of auxin-responsive genes. Science Signaling. 2014 7(328): ra53
20. Zhang H, Tang K, Qian W, Duan CG, Wang B, Zhang H, Wang P, Zhu X, Lang Z, Yang Y, Zhu JK. An Rrp6-like protein positively regulates noncoding RNA levels and DNA methylation in Arabidopsis. Molecular Cell. 2014 May 8;54(3):418-30.
21. Qian W, Miki D, Zhang H, Liu Y, Zhang X, Tang K, Kan Y, La H, Li X, Li S, Zhu X, Shi X, Zhang K, Pontes O, Chen X, Liu R, Gong Z, Zhu JK. A histone acetyltransferase regulates active DNA demethylation in Arabidopsis. Science. 2012, 336(6087): 1445-1448.
22. Zhu X, Pattathil, S, Mazumder K, Brehm A, Hahn, MG, Dinesh-Kumar SP and Joshi CP. Virus-induced gene silencing offers a functional genomics platform for studying plant cell wall formation. Molecular Plant. 2010, 3(5): 818-33 (Cover).
23. Zhu X, Caplan J, Mamillapalli P, Czymmek K, Dinesh-Kumar, SP. Function of endoplasmic reticulum calcium ATPase in innate immunity-mediated programmed cell death. EMBO Journal. 2010, 29: 1007-1018.
24. Caplan J, Zhu X (Co-first author), Mamillapalli P, Marathe R, Anandalakshmi R and Dinesh-Kumar SP. Induced ER chaperones regulate a receptor-like kinase to mediate antiviral innate immune response in plants. Cell Host & Microbe. 2009, 6(5): 457-469.
25. Zhu X, Dinesh-Kumar SP. Virus-induced gene silencing as a tool to identify host genes affecting viral pathogenicity. Methods in Molecular Biology. 2008, 451: 641-8.
26. Stepansky A, Less H, Angelovici R, Aharon R, Zhu X, Galili G. Lysine catabolism, an effective versatile regulator of lysine level in plants. Amino Acids. 2006; 30(2): 121-5.
27. Zhu X, Galili G. Lysine metabolism is concurrently regulated by synthesis and catabolism in both reproductive and vegetative tissues. Plant Physiology. 2004,135(1): 129-136.
28. Zhu X, Galili G. Increased lysine synthesis coupled with a knockout of its catabolism synergistically boosts lysine content and also transregulates the metabolism of other amino acids in Arabidopsis seeds. Plant Cell. 2003, 15(4): 845-853.
29. Zhu X, Tang G, Galili G. The activity of the Arabidopsis bifunctional lysine-ketoglutarate reductase/saccharopine dehydrogenase enzyme of lysine catabolism is regulated by functional interaction between its two enzyme domains. Journal of Biological Chemistry. 2002, 277(51): 49655-49661.
30. Tang G, Zhu X (Co-first author), Gakiere B, Levanony H, Kahana A, Galili G. The bifunctional LKR/SDH locus of plants also encodes a highly active monofunctional lysine-ketoglutarate reductase using a polyadenylation signal located within an intron. Plant Physiology. 2002, 130(1): 147-154.
31. Zhu X, Tang G, Granier F, Bouchez D, Galili G. A T-DNA insertion knockout of the bifunctional lysine-ketoglutarate reductase/saccharopine dehydrogenase gene elevates lysine levels in Arabidopsis seeds. Plant Physiology. 2001, 126(4): 1539-1545.
32. Zhu X, Tang G, Galili G. Characterization of the two saccharopine dehydrogenase isozymes of lysine catabolism encoded by the single composite AtLKR/SDH locus of Arabidopsis. Plant Physiology. 2000, 124(3): 1363-1372.
33. Zhu X, Tang G, Galili G. The catabolic function of the a-aminoadipic acid pathway in plants is associated with unidirectional activity of lysine-ketoglutarate reductase but not saccharopine dehydrogenase. Biochemical Journal. 2000, 351: 215-220.
34. Galili G, Tang G, Zhu X, Gakiere B: Lysine catabolism: A stress and development super- regulated metabolic pathway. Current Opinion in Plant Biology. 2001, 4(3): 261-266.
讲授课程：
1.高级生物化学 （全英课程，本科生）
2.植物学文献阅读（硕、博士生）
3.实验技能（硕、博士生）
